Human somatotropin competed for 125I-human somatotropin binding to hepatocytes from female or male rats. Bovine somatotropin and prolactin each inhibited part, but not all, of the uptake of 125I-human somatotropin. The binding of 125I-prolactin was inhibited by human somatotropin and prolactin, but not by bovine somatotropin. Bovine somatotropin and human somatotropin, but not prolactin, compet...

Use of bovine somatotropin to increase product ion of milk and milk solids poses the problem of allocation within herd and implications for genetic evaluatat ion records with and without bovine somatotropin were simulated for different correlations between performance with and without t reatment and response to it. Resulting within-herd variances and accuracy and bias in sire evaluations were c...

Bovine somatotropin (bST) has been isolated from pituitary glands and compared in a variety of chemical analyses and bioassays with somatotropin derived from recombinant Escherichia coli. Comparison of pituitary extracts and purified bST by Western blot analysis of two-dimensional gels suggested that the immunoreactive somatotropin species present in the extract were also present in the purifie...

Accession Number. U15731. Species. Bos taurus. Origin of Clone. Hybridizing with a 220-bp cDNA of the somatotropin receptor (Hauser et al., 1990) 10 clones were identified from a bovine genomic library (EMBL3 SP6pT7 cloning vector; Clontech Laboratories, Palo Alto, CA). A 2.9-kb XhoIIAatII fragment containing the promoter and exon I was subcloned into pGEM-7Zf (Promega, Madison, WI), and both s...

The isolation of growth hormone (somatotropin) from human, monkey, and whale pituitary glands has recently been reported (l-3). Preliminary studies on the hormone proteins from these species have suggested that phenylalanine is their sole N-terminal amino acid residue (l-3), in contrast to the two N-terminal residues, alanine and phenylalanine, which have been reported for the bovine somatotrop...

Bovine somatotropin (bST) increases milk production in dairy animals. The maximum increase in milk production has been observed up to 41%. Somatotropin did not change milk composition significantly. During short-term application of bST, cows mobilized their body-reserves to support the increased milk production. Although DM intake of cows was increased in the long-term applications, however, gr...

Recombinant bovine somatotropin (rBST) changes metabolism to spare glucose for milk synthesis in cows. Ceramides inhibit insulin responsiveness adipocytes and are associated with resistance production The mechanisms by which rBST supports lactation may involve ceramide. Eight multiparous lactating Holstein cows were enrolled a 2 × replicated Latin square design 14-d periods. Cows received singl...